Assessment of the performance of cruise ship waste management: Case study of the Rio de Janeiro port, Brazil.

The significant growth of the cruise ship industry has resulted in economic benefits, but there are also waste management-related challenges. The aim of this case study was to contribute to the research of cruise ship waste management at the Port of Rio de Janeiro. A3 methodology, applied for the first time in this context, was used to create questionnaires to assess the stakeholders involved, revealing that institutional bureaucracy has a negative effect on waste management. In addition, secondary data on unloaded waste, obtained from the transport manifests, showed that 56 % contained reliable information. The main wastes were plastics (57 %), glass (17 %), metal (9 %), and others. With respect to destinations, 41 % were sent to landfills, 55 % recycled and 4 % thermally treated. Only 5 % of cruise ship moorings removed waste at the Port of Rio de Janeiro, attributed to factors such as high costs.

Cellular Growth Arrest and Efflux Pumps Are Associated With Antibiotic Persisters in Streptococcus pyogenes Induced in Biofilm-Like Environments.

Streptococcus pyogenes (group A Streptococcus-GAS) is an important pathogen for humans. GAS has been associated with severe and invasive diseases. Despite the fact that these bacteria remain universally susceptible to penicillin, therapeutic failures have been reported in some GAS infections. Many hypotheses have been proposed to explain these antibiotic-unresponsive infections; however, none of them have fully elucidated this phenomenon. In this study, we show that GAS strains have the ability to form antimicrobial persisters when inoculated on abiotic surfaces to form a film of bacterial agglomerates (biofilm-like environment). Our data suggest that efflux pumps were possibly involved in this phenomenon. In fact, gene expression assays by real-time qRT-PCR showed upregulation of some genes associated with efflux pumps in persisters arising in the presence of penicillin. Phenotypic reversion assay and whole-genome sequencing indicated that this event was due to non-inherited resistance mechanisms. The persister cells showed downregulation of genes associated with protein biosynthesis and cell growth, as demonstrated by gene expression assays. Moreover, the proteomic analysis revealed that susceptible cells express higher levels of ribosome proteins. It is remarkable that previous studies have reported the recovery of S. pyogenes viable cells from tissue biopsies of patients presented with GAS invasive infections and submitted to therapy with antibiotics. The persistence phenomenon described herein brings new insights into the origin of therapeutic failures in S. pyogenes infections. Multifactorial mechanisms involving protein synthesis inhibition, cell growth impairment and efflux pumps seem to play roles in the formation of antimicrobial persisters in S. pyogenes.

Efficiency in the environmental management of plastic wastes at Brazilian ports based on data envelopment analysis.

The aim of this study was to analyze different port areas (leased, nonleased and vessels) in terms of plastic segregation (scenario 1) and how much of this plastic is recycled (scenario 2). Data envelopment analysis was applied and the variables were total amount of solid waste and percentage of segregated plastic in relation to total solid waste (scenario 1) and amount of segregated plastics and percentage of recycled in relation to segregated plastics (scenario 2). Segregation efficiency was low (49%) in the nonleased area, but all the segregated material is recycled, suggesting that the management bottleneck in this case is waste segregation. Similar segregation results were obtained in the leased areas and vessels (36 and 35%, respectively), but recycling efficiency was greater in the former (92 and 24%, respectively).